Coding

Part:BBa_K1439004:Experience

Designed by: Zhaoliang Chen   Group: iGEM14_OUC-China   (2014-10-05)

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Applications of BBa_K1439004

User Reviews

UNIQ7180d6239f63e04e-partinfo-00000000-QINU UNIQ7180d6239f63e04e-partinfo-00000001-QINU OUC-China K1439004 Experience1.png

Fig 1.

Fig 1.We wanted to know if the fusion protein will combine the plasimd. We mixed up the protein TAT:H4 with the plasmid pcDNA3.1(+),and the mass ratios of DNA and protein were 1:0,1:1, 1:2, 1:4, 1:6, 1:8 in 2ul glucose solution. This picture showed that TAT-H4 can combine with DNA, because the plasmid was retraded. OUC-China K1439004 Experience2.png

Fig 2.

Fig2.We wanted to test if the fusion protein can protect the plasmid, we used DNaseI deal with the complex that the protein TAT:H4 with the plasmid pcDNA3.1(+),and the mass ratios of DNA and protein were 1:0,1:1, 1:2, 1:4, 1:6, 1:8 in 2ul glucose solution. This picture shows that the fusion protein can protect the plasmid.